ELISA Plate Operation Process

The ELISA plate operation process generally includes the following steps:

1. Pretreatment of the plate: The wells of the plate are cleaned and treated to remove any contamination and impurities.

2. Sample Addition: The sample to be tested is added to the wells of the plate, usually requiring some dilution.

3. Detection Reagent Addition: The appropriate reagents for the analyte are added, such as antibodies and substrates.

4. Reaction: The sample and reagents are allowed to fully react, usually requiring a certain incubation period.

5. Measurement: The concentration of the analyte in the sample is quantified by measuring parameters such as absorbance, fluorescence, or color reaction intensity.